【摘要】 目的探讨缓释血管内皮生长因子复合支架与脂肪干细胞构建工程化脂肪的可行性。方法制备VEGF-PLGA纳米微球缓释支架,检测支架释放VEGF浓度,体外提取培养ADSCs,检测复合支架对ASC生长增殖的影响。将复合支架和ADSCs植入裸小鼠背部,8周后切取植入物称重,组织切片HE染色,评估效果。结果 VEGF-PLGA纳米微球缓释复合支架能连续12d释放较高浓度的VEGF,对ADSCs的生长增殖无影响。动物实验结果显示复合支架与ADSCs共移植能显著提高脂肪组织的形成,增加血管的生成(P<0.01),减少组织坏死。结论 VEGF-PLGA纳米微球缓释系统具有良好的生物安全性,其与ADSCs共移植构建工程化脂肪组织具有一定的可行性。更多还原

【Abstract】 Objective To investigate the feasibility of construction of engineered adipose tissue with sustained-release vascular endothelial growth factor(VEGF) composite stent and adipose stem cells.Methods VEGF-PLGA nanospheres sustained-release scaffolds were prepared and the concentration of VEGF was measured. ADSCs, was extracted in vitro to detect the effect of composite scaffolds on the growth and proliferation of ASC.The composite scaffold and ADSCs were implanted into the back of nude mice. After 8 weeks, the implants were weighed, and the tissue sections were stained with HE to evaluate the effect. Results VEGF-PLGA nanospheres sustained-release composite scaffolds could release higher concentration of VEGF, for 12 d without any effect on the growth and proliferation of ADSCs.The results of animal experiments showed that co-transplantation of composite scaffold and ADSCs could significantly increase the formation of adipose tissue, increase angiogenesis(P<0. 01), and reduce tissue necrosis.Conclusion VEGF-PLGA nanospheres sustained-release system has good biological safety and it is feasible to co-transplant with ADSCs to construct engineered adipose tissue.更多还原