【摘要】 目的考察盐制对泽泻中总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量的影响。方法采用紫外分光光度计检测（UV）法测定生泽泻和盐泽泻中总泽泻醇的含量,显色剂为间硝基苯和氢氧化钾,检测波长545nm;采用HPLC法测定生泽泻和盐泽泻中24-乙酰泽泻醇A和23-乙酰泽泻醇B的含量,色谱柱为依利特C₁₈柱（4.6mm×250mm,5μm）,流动相为乙腈-水梯度洗脱,检测波长208nm,柱温25℃,流速1ml/min。结果 UV法和HPLC法中,总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B在考察的线性范围内,线性关系良好（r>0.999 0）;在生泽泻和盐泽泻中的平均回收率为97.81%¹00.05%,RSD<3%。三者在10批生泽泻样品中的平均含量分别为4.41%、0.208%、0.065%;在10批盐泽泻样品中的平均含量分别为9.62%、0.232%、0.017%。结论盐制对泽泻中总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量影响较大,其中总泽泻醇的含量明显增加,24-乙酰泽泻醇A的含量略有增加,而23-乙酰泽泻醇B的含量明显降低。更多还原

【Abstract】 Objective To observe the effect of salted processing on the contents of total alismol,24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma.Methods UV was used for the determination of total alismol′s content in crude Alisma Rhizoma and salted Alisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers.The detection wavelength was 545 nm.HPLC was used for the determination of 24-acetylalisol A′s and 23-acetylalisol B′s contents in crude Alisma Rhizoma and salted Alisma Rhizoma with Elite C₁₈（4.6 mm×250 mm,5μm）as the chromatographic column.The mobile phase was gradient elution with acetonitrile and water.The detection wavelength was 208 nm.The column temperature was set at 25℃.The flow rate was 1 ml/min.Results The linear relationships of total alismol,24-acetylalisol A and 23-acetylalisol B were fine（r>0.999 0）in the linear ranges by UV and HPLC.The average recoveries of them in crude Alisma Rhizoma and salted Alisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%.The average contents of total alismol,24-acetylalisol A and 23-acetylalisol B in ten batches of crude AlismaRhizoma were 4.41%,0.208%,0.065%respectively.The average contents of them in ten batches of salted Alisma Rhizoma were 9.62%,0.232%,0.017% respectively.ConclusionThe contents of total alismol,24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma were influenced considerably by salted processing.The content of total alismol increased obviously.The content of 24-acetylalisol A increased slightly.The content of 23-acetylalisol B decreased obviously.更多还原