【摘要】 目的研究青藏高原野生型鼠疫噬菌体对鼠疫诊断噬菌体耐受株的特异性。方法 18株野生型鼠疫噬菌体通过富集、分纯后,利用双层琼脂平板法检测其对实验室诱导的3株鼠疫诊断噬菌体耐受株的特异性裂解能力。结果分离自青藏高原鼠疫疫源地的18株野生型鼠疫噬菌体对典型鼠疫弱毒菌EV 76和0614F均能完全裂解,对R-0614F-10代菌株不完全裂解,对R-0614F-38代、R-0614F-43代不裂解,这与鼠疫诊断噬菌体Yep-phi对R-0614F-10代、R-0614F-38代、R-0614F-43代的裂解能力一致。选取3株野生型鼠疫噬菌体作用于鼠疫诊断噬菌体耐受株R-0614F-12代,形成的噬菌斑大小不一致,直径为2~10 mm,边缘不整齐,中心有再生菌生长。结论 18株野生型鼠疫噬菌体对3株鼠疫诊断噬菌体耐受株裂解作用的特异性可能与鼠疫菌细胞壁受体结构和菌株基因组序列发生改变有关。更多还原

【Abstract】 Objective To study the specificity of wild-type Yersinia pestis bacteriophage in the Tibetan Plateau for the diagnosis of phage-resistant strains of plague. Methods After the 18 wild-type Yersinia pestis were enriched and purified,the specific lysis ability of the three laboratory-induced diagnostic phage-resistant strains of plague was detected by double agar plates. Results 18 wild-type plague bacteriophages isolated from the plague foci of the Qinghai-Tibet plateau were able to completely lyse the typical attenuated EV 76 and 0614 F virulence strains,incompletely lysing the R-0614 F-10 strain,and R-0614 F-38,R-0614 F-43 generation did not cleave,which was consistent with the lysis ability of the Yersinia pestis diagnostic Yep-phi to R-0614 F-10,R-0614 F-38,and R-0614 F-43 generations. Three wild-type Yersinia pestis bacteriophages were selected to act on plague-diagnosed phage-tolerant strain R-0614 F-12 generation. The size of plaque formed was inconsistent. The diameter was 2 ~ 10 mm, the edges were irregular, and the regenerating bacteria grew in the center. Conclusion The specificity of the 18 strains of wild-type Yersinia pestis bacteriophage for the lysis of 3 plague-diagnosed phage-resistant strains may be related to changes in the structure of the Yersinia pestis cell wall receptor and the genome sequence of the strain.更多还原