【摘要】 目的采用SD大鼠高脂高糖饮食联合腹腔注射链脲菌素（STZ）造模,观察糖尿病大鼠的胃排空功能,探索建立糖尿病胃轻瘫（DGP）大鼠模型的方法。方法采用高脂高糖饲料（45%脂肪）喂养2周,禁食、自由饮水12 h后腹腔注射50 mg·kg^-1 STZ的方法建立糖尿病大鼠模型。随机选择10只正常SD大鼠作为正常组,10只造模成功的SD大鼠作为模型组。两组大鼠均予普通饲料喂养8周后取鼠胃,检测胃排空及小肠推进率。结果模型组大鼠形体消瘦,体重减轻,摄食增加。与正常组比较,模型组大鼠血糖水平均明显升高（P<0.05）,胃残留物的重量明显增加（P <0.05）,胃排空率显著下降。模型组大鼠小肠推进率与血糖呈负相关（相关系数r=-0.46,P=0.041）。结论高脂高糖饮食联合STZ腹腔注射的方法建立糖尿病大鼠模型后普通饲料喂养8周可成功建立DGP大鼠模型,可成为DGP动物实验研究经济、简便、易行的造模方法。更多还原

【Abstract】 Objective To observe the gastric motility of diabetic rats and to explore a method of establishing a diabetic gastroparesis(DGP) rat model by feeding diabetic rats with high fat and high sugar diet combined with intraperitoneal injection of STZ. Methods To establish diabetic rat model, normal SD rats were fed with high fat and high sugar diet(45% fat content) for 2 weeks; then streptozocin(STZ) 50 mg·kg-1 was intraperitoneally injected after fasting and free drinking water for 12 hours. Ten normal SD rats were randomly selected for normal group, and 10 SD rats modeled successfully for model group. Stomachs of rats in the 2 groups were taken after 8 weeks of common feeding to measure gastric emptying and intestinal propulsion rate. Results Rats in the model group had thin physique, weight loss, and increased food intake. Compared with the normal group, the blood glucose level of the model group was significantly higher(P <0.05), the weight of the gastric residue was significantly increased(P<0.05), and the gastric emptying was significantly decreased. There was a negative correlation between the rate of intestinal propulsion in the model group(correlation coefficient r=-0.46, P =0.041). Conclusion Diabetic rat modeled by administrating high fat and high sugar diet feeding combined with STZ intraperitoneal injection and then administrating normal feeding for 8 weeks can establish a DGP rat model, which can be an economical, simple, and feasible modeling method for DGP animal experiments.更多还原