【摘要】 目的:建立山楂中6个黄酮类物质的液相色谱-串联质谱（LC-MS/MS）测定方法。方法:实验优化了液相色谱条件和质谱参数。采用C₁₈柱分离,流动相为乙腈-水（含0.1%甲酸）,梯度洗脱,流速0.2 m L·min^-1,以电喷雾离子源正离子多反应监测（MRM）模式进行MS/MS检测。结果:6个黄酮类物质在各自的线性范围内具有良好的线性关系,相关系数大于0.994,低、中、高3个添加水平的平均回收率在80.4%¹05.0%之间,RSD小于10%。10批样品中6个黄酮成分的含量测定结果:儿茶素,0.03⁵.39μg·g^-1;牡荆素,0.11¹.02μg·g^-1;木犀草素,0.02⁰.09μg·g^-1;槲皮素,3.72¹4.02μg·g^-1;芦丁,3.32⁵.79μg·g^-1;金丝桃苷,31.32⁸0.89μg·g^-1。结论:该方法可用于测定山楂中儿茶素、牡荆素、槲皮素、木犀草素、芦丁、金丝桃苷。更多还原

【Abstract】 Objective:To establish a high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）method for simultaneous determination of 6 functional components in hawthorn.Methods:The conditions of chromatography and mass spectrometry were optimized.The 6 flavonoids were separated on a C₁₈chromatographic column with acetonitrile and 0.1%formic acid as mobile phase under gradient elution at a flow rate of 0.2 m L·min^-1.The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring（MRM）mode.Results:Good linearities for all the compounds,with correlation coefficients over 0.994,were acquired.The recoveries（n=6）were in the range of 80.4%to 105.0%,while RSDs were less than 10%.The contents of 6 functional components in 10 batches of samples were as follows:catechin,0.03-5.39μg·g^-1;vitexin,0.11-1.02μg·g^-1;luteolin,0.02-0.09μg·g^-1;quercetin,3.72-14.02μg·g^-1;rutin,3.32-5.79μg·g^-1;hyperoside,31.32-80.89μg·g^-1.Conclusion:The established method can be used to determine catechin,vitexin,quercetin,luteolin,rutin and hyperoside in hawthorn.更多还原