【摘要】 建立高效液相色谱—双波长同时检测咖啡豆提取物中绿原酸和咖啡因含量的方法。咖啡因和绿原酸在0.2～1.0 mg·mL^-1范围内,峰面积与浓度呈现良好的线性关系。咖啡因:Y=44077X+227.78,R=0.9997,RSD=0.78%;绿原酸:Y=34896X+289.96,R=0.9995,RSD=0.93%。以绿原酸含量为指标,在单因素试验基础上建立正交试验,确定从绿咖啡豆中提取绿原酸的最佳条件为乙醇浓度20%,液料比6∶1,提取时间60 min,提取温度70℃。以咖啡因和绿原酸含量为指标,通过萃取试验,确定最佳脱咖啡因萃取剂为环己烷-三氯甲烷-丙酮混合溶剂,萃取工艺为1.5倍体积萃取剂萃取3次,产物中咖啡因含量由（3.52±0.08）%下降至（0.47±0.11）%。更多还原

【Abstract】 The method of high performance liquid chromatography with dual wavelength was established for simultaneous determination of caffeine and chlorogenic acid in coffee bean extracts. The result showed a good linear relationship between peak area and concentration in the range of 0.2—1.0 mg·mL^-1. The regression equations of caffeine were: Y=44077X+227.78, R=0.9997, RSD=0.78%; the regression equations of chlorogenic acid were: Y=34896X+289.96, R=0.9995, RSD=0.93%. With chlorogenic acid content as indicator, the optimal conditions for extracting chlorogenic acid from green coffee beans were determined as follows: ethanol concentration was 20%, solid-liquid ratio was 6:1, extraction time was 60 min, the extraction temperature was 70 ℃according to orthogonal test and single factor test. Taking caffeine and chlorogenic acid contents as indicators, the best decaffeination extractant was cyclohexane-trichloromethane-acetone mixture, and the extraction process was extracted three times with 1.5 volumes of cyclohexane-trichloromethaneacetone mixture with the caffeine content decreased from（3.52±0.08）% to（0.47±0.11）%.更多还原