【摘要】 【目的】寻找与山羊肉髯相关的基因单核苷酸多态性(single nucleotide polymorphism,SNP)位点,分析肉髯性状与体重、体尺和繁殖性能的相关性。【方法】以南江黄羊为试验材料,对肉髯性状候选基因区域(Chr10:25 Mb~28 Mb)的15个SNP位点进行检测分析;测量肉髯长度和周长,观察肉髯切片的组织形态;进一步比较分析有肉髯个体和无肉髯个体之间的体重体尺性状(包括体重、体长、体高和胸围)和繁殖性状(包括初产和经产母羊产羔率)的差异。【结果】南江黄羊Chr 10:25 Mb~28 Mb区域内,仍未发现与肉髯性状显著相关的SNP位点。两侧肉髯的长度之间、周长之间均呈显著正相关(P<0.01),但单侧肉髯长度与周长并无显著性相关(P>0.05)。在生长发育早期,肉髯个体的体长和体高较小(P<0.01),到成年后差异不显著(P>0.05);同时,肉髯个体的体重和胸围也更小(P<0.01),尤其是在6月龄至成年后。而有肉髯和无肉髯初产和经产母羊的产羔率差异均不显著(P>0.05)。【结论】在山羊肉髯性状候选基因区域内未发现与肉髯性状显著相关的SNP位点;有肉髯山羊的体重和体尺均小于无肉髯山羊,但肉髯对母羊产羔率无显著影响。更多还原

【Abstract】 [Purpose]To investigate gene mutation loci related to goat wattles and the effect of wattle on growth and reproduction traits of Nanjiang yellow goat population.[ Methods] Single nucleotide polymorphisms(SNPs) were detected on candidate genes located in Chr 10: 25 Mb-28 Mb.Meanwhile, wattles were sliced and stained to observe its histological structures. We analyzed the correlation between the wattle length and perimeter, and the differences of growth traits including body weight, body length, body height and chest girth, as well as reproductive traits between goats with or without wattles.[Results]We failed to confirm any SNP associated with wattles directly. Interestingly, the wattles length located on both sides were significantly positively correlated with each other,so did wattle perimeter(P<0.01), but there was no significant correlation between the length and perimeter(P>0.05). Generally, compared with non-wattle goats, goats with wattles possessed smaller body length and body height especially at newborn(P<0.01), while goats with wattles had smaller chest girth and body weight particularly in later development stage(P<0.01). Nevertheless, the kidding percentage including the first time and the latter showed no significant difference between does with or without wattles(P>0.05).[ Conclusion] No significant SNP associated with wattle was found in candidate gene area of goat. Wattles are tended to link with lower growth rate of goat mainly embodying in body weight and body size, but wattle trait has insignificant effect on the kidding percentage.更多还原