【摘要】 目的:探讨福州地区汉族人群强直性脊柱炎(AS)患者人类白细胞抗原B27(HLA-B27)基因亚型分布及其临床意义。方法:采用流式细胞术检测AS患者血液HLA-B27的表达,同时用荧光定量PCR染料法检测其基因亚型,分析HLA-B27基因亚型与病例资料的关系。结果:202例AS患者中,流式细胞术检出HLA-B27阳性194例,阳性率96.03%,荧光定量PCR染料法检出HLA-B27阳性196例,阳性率97.03%,比较差异无统计学意义(P>0.05)。共检出HLA-B27基因亚型4种,其中HLA-B2704为88.61%,2705/07为5.45%。结论:荧光定量PCR法和流式细胞术法检测HLA-B27的阳性率相近,福州地区汉族人群AS患者以HLA-B2704和2705/07亚型为主,携带这两种亚型的AS患者首发临床表现均为骶髂关节炎和外周关节炎。更多还原

【Abstract】 Objective:To investigate the distribution and clinical significance of human leukocyte antigen B27(HLA-B27) genotype in ankylosing spondylitis(AS) patients in Fuzhou Han population.Method:Flow cytometry was used to detect the expression of HLA-B27 in AS patients,and fluorescence quantitative PCR was used to detect the genotype of HLA-B27.The relationship between HLA-B27 gene subtypes and case data was analyzed.Result:Among 202 AS patients,194 were HLA-B27 positive by flow cytometry(96.03%) and 196 were HLA-B27 positive by fluorescence quantitative PCR(97.03%),there was no significant difference between the two methods in detecting the positive rate of HLA-B27(P>0.05).A total of 4 HLA-B27 subtypes were detected,of which HLA-B 2704 was 88.61% and 2705/07 was 5.45%.Conclusion:There is no significant difference in the positive rate of HLA-B27 detected by fluorescence quantitative PCR and flow cytometry.HLA-B2704 and 2705/07 subtypes are predominant in AS patients of Han nationality in Fuzhou.The first clinical symptom of AS patients carrying these two subtypes are sacroiliac arthritis and peripheral arthritis.更多还原