【摘要】 目的探究老鼠簕生物碱A对环磷酰胺致小鼠生殖系统损伤的保护作用。方法将40只小鼠随机分成5组:阴性对照组(生理盐水组)、阳性对照组(环磷酰胺组)、老鼠簕生物碱A高剂量组(200 mg/kg)、老鼠簕生物碱A中剂量组(100mg/kg)、老鼠簕生物碱A低剂量组(50mg/kg),每组8只。3d适应喂养后,阴性对照组和阳性对照组每只每天灌胃0.4ml的生理盐水。老鼠簕生物碱A高、中、低剂量组按每公斤体重每天予老鼠簕生物碱A灌胃。每天上午9点开始灌胃,每组连续灌胃14d,第13d给药后,除阴性对照组外,各组小鼠按每公斤体重每天腹腔注射200 mg/kg环磷酰胺造模,连续2d。末次给环磷酰胺24h后,立即将小鼠处死,取其睾丸,观察睾丸细胞组织切片;观察睾丸细胞微核数;观察精子畸形数。结果 (1)组织切片观察结果显示,与阴性对照组相比,阳性对照组、老鼠簕生物碱A各组小鼠睾丸组织结构都出现了病理改变。而老鼠簕生物碱A各组与阳性对照组相比,阳性对照组出现了严重的睾丸组织细胞病理改变,随着老鼠簕生物碱A剂量的增加,病理改变越来越少。(2)与阴性对照组相比,阳性对照组、老鼠簕生物碱A各组小鼠睾丸细胞的微核率、精子畸形率均高于阴性对照组(P<0.01或P<0.05);与阳性对照组相比,老鼠簕生物碱A各组小鼠睾丸细胞的微核率、精子畸形率均低于阳性对照组(P<0.01),且随着给药剂量的增加,微核率以及精子畸形率均降低。结论在适当剂量下,老鼠簕生物碱A对环磷酰胺致小鼠生殖系统损伤有不同程度的保护作用。更多还原

【Abstract】 Objective To explore the protective effect of Acanthus ilicifolius alkaloids A on the damage of the reproductive system in mouse induced by cyclophosphamide. Methods A total of 40 mice were randomly divided into five groups:a negative control group(normal saline group),apositive control group(cyclophosphamide group),a high-dose Acanthus ilicifolius alkaloids A group(200 mg/kg),a medium-dose Acanthus ilicifolius alkaloids A group(100 mg/kg)and a low-dose Acanthus ilicifolius alkaloids A group(50 mg/kg),8 mice in each group.After three days of adaptive feeding,mice in the negative control group and positive control group received intragastric administration of 0.4 ml saline every day,mice in high-,medium-and low-dose Acanthus ilicifolius alkaloids A groups received intragastric administration of Acanthus ilicifolius alkaloids A every day according to per kilogram of bodyweight.Intragastric administration started at 9 am every day for 14 consecutive days for each group.On the 13 th day after the intragastric administration of Acanthus ilicifolius al-kaloids A or saline was completed,except the negative control group all groups received intraperitoneal injection of cyclophosphamide(200 mg/kg)according to per kilogram of bodyweight for 2 consecutive days for developing the models.Twenty-four hours after the last intraperitoneal injection of cyclophosphamide,the mice were immediately killed,then their testicles were taken out.The testicular cell tissue slices,the testicular cells micronucleus number and the sperm malformation number were observed. Results(1)The tissue slices observation results showed that compared with the negative control group,the testicular tissue structures of the mice appeared pathological changes in positive control group and in Acanthus ilicifolius alkaloids A groups.Compared Acanthus ilicifolius alkaloids A groups with the positive control group,there were serious pathological changes in the positive control group.With the Acanthus ilicifolius alkaloids A dose increase,the pathological changes in the Acanthus ilicifolius alkaloids A groups were less and less.(2)The micronucleus rate and sperm malformation rate of testicular cells in the positive control group and the Acanthus ilicifolius alkaloids A groups were higher than those of the negative control group(P <0.01 or P <0.05).The micronucleus rate and sperm malformation rate of testicular cells in the Acanthus ilicifolius alkaloids A groups were lower than those of the positive control group(P <0.01),and with the dosage increase,the micronucleus rate and sperm malformation rate were decreased. Conclusion At an appropriate dose,Acanthus ilicifolius alkaloids A has different degrees of protective effects on mouse reproductive system damage induced by cyclophosphamide.更多还原