【摘要】 目的探讨S100B在寻常型银屑病患者外周血单个核细胞(PBMC)中的表达水平及意义。方法选取2016年3月—2017年10月来我院诊治的35例寻常型银屑病患者(银屑病组)及17例正常对照(正常对照组)为研究对象,采用实时定量PCR检测两组外周血单个核细胞中S100B以及STAT3的mRNA表达,同时采用流式细胞仪检测两组PBMC中S100B蛋白表达水平,并采用皮尔逊相关系数分析S100B和银屑病皮损面积和严重指数评分(PASI)的相关性。结果银屑病组PBMC中S100B mRNA的相对表达量为(0.057±0.004),正常对照组为(0.043±0.003),两组比较差异有统计学意义(t=14.322,P<0.001)。银屑病组PBMC中STAT3 mRNA的相对表达量为(0.758±0.067),正常对照组为(0.418±0.035),两组比较差异有统计学意义(t=23.954,P<0.05)。皮尔逊相关系数分析显示S100B和PASI具有明显相关性。银屑病组PBMC中S100B蛋白表达水平较正常对照组明显增高。结论寻常型银屑病外周血单个核细胞S100B和STAT3表达异常升高,S100B可能通过调控STAT3参与了寻常型银屑病的发生发展。更多还原

【Abstract】 Objective To investigate the expression and significance of S100B in peripheral blood mononuclear cells(PBMC)from patients with psoriasis vulgaris. Methods Thirty-five patients with psoriasis vulgaris(psoriasis group)cared at our hospital from March 2016 to October 2017 and 17 normal controls(normal control group)were recruited in this research.A real-time PCR was used to detect the expressions of S100B mRNA and STAT3 mRNA in the peripheral blood mono-nuclear cells from both of the psoriasis group and the normal control group,meanwhile the S100B protein expression in PBMC of the two groups were detected by flow cytometry.Pearson correlation coefficient was applied to analyze correlation between S100B and psoriasis area and severity index(PASI). Results The relative expression of S100B mRNA in PBMC of the psoriasis group was(0.057±0.004),and it was(0.043±0.003)in the normal control group,comparison between the two groups showed that there was statistical difference(t =14.322,P <0.001).The relative expression of STAT3 mRNA in PBMC of the psoriasis group was(0.758±0.067),and it was(0.418±0.035)in the normal control group,comparison between the two groups showed that there was statistical difference(t=23.954,P<0.05).Pearson correlation coefficient analysis results showed that S100B had obvious association with PASI.The S100B protein expression in PBMC of the psoriasis group was significantly higher than that of the normal control group. Conclusion The expressions of S100 Band STAT3 in PBMC from patients with psoriasis vulgaris are abnormally elevated.S100B may be involved in the genesis and development of psoriasis vulgaris by regulating STAT3.更多还原