【摘要】 目的探讨重楼(Paris polyphylla)的醇提取物活性单体pp-22对人鼻咽癌CNE-1细胞的增殖、周期和凋亡的影响。方法采用MTT法,流式细胞术实验检测pp-22对人鼻咽癌CNE-1细胞的增殖、周期及凋亡的影响。结果重楼提取物活性单体pp-22能抑制CNE-1细胞的生长,并与时间、剂量呈正相关;流式细胞术检测显示,pp-22能导致人鼻咽癌CNE-1细胞发生G2/M期周期阻滞和凋亡。结论重楼醇提取物活性单体pp-22能够抑制CNE-1细胞增殖,引起G2/M期周期阻滞,诱导凋亡。更多还原

【Abstract】 Objective To investigate the effect of pp-22, a monomer purified from Paris polyphylla on the proliferation, cycle and apoptosis of human nasopharyngeal carcinoma cell CNE-1. Methods MTT, flow cytometry were used to check the proliferation, cycle and apoptosis of CNE-1 cells after treatment with different concentrations of pp-22. Results pp-22 inhibited CNE-1 cell proliferation in a concentration and time-dependent manner. Besides, pp-22 arrested CNE-1 cell cycle at G2/M phase and induced cell apoptosis. Conclusions The monomer purified from Paris polyphylla pp-22 can effectively inhibit cell proliferation and promote cell apoptosis in human CNE-1 cells.更多还原