【摘要】 目的探讨Toll样受体(TLR)在多发性骨髓瘤(MM)患者骨髓单个核细胞(BMMCs)中的表达水平及意义。方法选取MM患者30例作为观察组,另选取同期门诊骨髓报告为增生性贫血患者10例作为对照组,观察组患者采用硼替佐米联合地塞米松方案化疗。采用实时荧光定量法检测2组BMMCs中TLR表达水平,分析TLR与MM临床分期的关系。结果TLR-4和TLR-9在观察组BMMCs中相对表达量显著高于对照组(P<0.05);TLR-4和TLR-9在不同分期MM患者BMMCs中表达水平差异有统计学意义(P<0.05);TLR-4与M蛋白和β2-MG呈正相关(r=0.673、0.466,P=0.023、0.041),TLR-9与M蛋白和β2-MG呈正相关(r=0.547、0.424,P=0.031、0.048);治疗后,MM患者BMMCs中TLR-4和TLR-9相对表达量显著降低(P<0.05)。结论 TLR-4和TLR-9在MM患者BMMCs中表达量明显升高,与临床分期、M蛋白和β2-MG相关。更多还原

【Abstract】 Objective To investigate the expression of Toll-like receptor(TLR) in bone marrow mononuclear cells(BMMCs) of patients with multiple myeloma(MM) and its significance.Methods Totally 30 patients with MM were selected as observation group,and 10 patients with proliferative anemia were selected as control group. Patients in the observation group were treated with bortezomib combined with dexamethasone chemotherapy. The expression level of TLR in BMMCs was detected by real-time fluorescence quantitative method in both groups. The relationship between TLR and the clinical stages of MM was analyzed. Results The relative expression levels of TLR-4 and TLR-9 in BMMCs in the observation group were significantly higher than those in the control group(P < 0. 05). The expression levels of TLR-4 and TLR-9 in patients with different stages of MM showed significant differences(P < 0. 05). TLR-4 was positively correlated with M protein and β2-MG(r = 0. 673,0. 466,P = 0. 023,0. 041),and TLR-9 was also positively correlated with M protein and β2-MG(r = 0. 547,0. 424,P = 0. 031,0. 048). After treatment,the relative expression levels of TLR-4 and TLR-9 in BMMCs in patients with MM decreased significantly(P < 0. 05). Conclusion The expression levels of TLR-4 and TLR-9 are obviously higher in patients with MM,which is related to the clinical stage,M protein and β2-MG.更多还原