【摘要】 目的:研究急性白血病(AL)患儿血清转铁蛋白受体(serum transferrin receptor,s TFR)的表达变化及其相关作用机制。方法:选取2016年6月至2017年6月在我院接受治疗的46急性白血病患儿纳入AL组,并募集同期体检健康儿童40例纳入对照组。记录患者的相关临床资料,分析血清转铁蛋白受体与患者临床特征的相关性。KG-1a和TCHu147细胞的TFR基因经RNA干扰技术沉默后,采用MTT法、流式细胞术检测TFR基因对KG-1a细胞和TCHu147细胞增殖、细胞周期的作用。采用Western blot法检测白血病细胞经siRNA干扰后相关细胞周期蛋白水平。结果:AL组s TFR水平显著高于对照组(P <0. 05),外周血白血病细胞中TFR的mRNA水平及TFR蛋白表达量均高于对照组(P <0. 05)。s TFR水平与AL患儿的白细胞数、白血病细胞比例、白血病细胞绝对数、铁调素(Hepc)和危险等级等指标密切相关(P <0. 05)。经TFR siRNA干扰后的KG-1a和TCHu147细胞增殖能力则受到明显抑制(P <0. 05)。流式细胞术检测显示,转染TFR siRNA后,KG-1a和TCHu147细胞的G0/G1期比例分别为(62. 51±5. 39)%、(63. 37±4. 27)%,与对照组(空白对照和阴性对照)相比均显著增高(P <0. 05); KG-1a和TCHu147细胞的G2/M期的比例为(5. 74±1. 34)%、(7. 37±1. 56)%,与对照组(空白对照和阴性对照)相比均显著下降(P <0. 05)。TFR siRNA转染后的KG-1a和TCHu147细胞中Cyclin E和CDK2表达水平显著下降(P <0. 05); TFR siRNA转染后的KG-1a和TCHu147细胞中P27表达水平显著上升(P <0. 05)。结论:AL患儿外周血白血病细胞可合成较多的TFR蛋白,转运至细胞外后导致s TFR水平上升,通过下调TFR基因的表达能有效干扰白血病细胞分裂。更多还原

【Abstract】 Objective: To investigate the expression changes of serum transferrin receptor( s TFR) and its related mechanism in children with acute leukemia( AL). Methods: Forty-six children with acute leukemia treated in our hospital from June 2016 to June 2017 w ere selected and enrolled in the AL group,40 healthy children w ere enrolled in the control group. The related clinical data w ere recorded,including age,sex and CNSL level. RNA interference technology was used to silence TFR genes of KG-1 a and TCHu147 cells,MTT method and flow cytometry w ere used to analyze the effect of TFR gene on proliferation and cell cycle of KG-1 a cells and TCHu147 cells. Western blot was used to detect the level of cyclin related to leukemic cells after siRNA interference. Results: The level of s TFR in AL patients was significantly higher than that of healthy people( P < 0. 05). The mRNA and protein expression levels of TFR in peripheral blood leukemic cells w ere all higher than those in healthy people( P < 0. 05). The level of s TFR closely related to the white blood cell( WBC) count,the proportion and absolute number of leukemic cells,hepcidin( Hepc)level,and risk grade in AL patients( P < 0. 05). The proliferation ability of KG-1 a and TCHu147 cells after TFR siRNA interference was significantly inhibited( P < 0. 05). Fow cytometry show ed that after the TFR siRNA interference,the ratio of KG-1 a and TCHu147 cells in G0/G1 phase was 62. 51% ± 5. 39% and 63. 37% ± 4. 27%,respectively,which increased significantly as compared with the blank and negative control group( P < 0. 05); the ratio of KG-1 a and TCHu147 in G2/Mphase was 5. 74% ± 1. 34% and 7. 37% ± 1. 56%,respectively,which significantly decreased as compared with the blank control and the negative control group( P < 0. 05). Conclusion: The peripheral blood leukemic cells of AL patients can synthesize more TFR protein,lead into the increase of s TFR level. It can effectively interfere the division of leukemia cells by dow nregulating the expression of TFR gene.更多还原