【摘要】 目的:探讨人工合成长链多聚磷酸盐对凝血和血小板聚集的影响。方法:通过凝血常规检测、凝血因子活力检测和血小板聚集实验,检测人工合成长链多聚磷酸盐对凝血和血小板聚集的影响,采用ELISA、流式细胞术、高内涵成像技术探讨长链多聚磷酸钠盐的作用机制。结果:长链多聚磷酸盐可延长活化部分凝血活酶时间,降低凝血因子FⅧ、FⅨ、FⅪ、FⅫ活力,阻滞ADP诱导的血小板聚集,降低血小板内钙离子及TXA2浓度。结论:人工合成长链多聚磷酸盐可抑制内源性凝血并抑制血小板聚集,其机制可能与抑制血小板内钙离子及TXA2有关。更多还原

【Abstract】 Objective: To investigate the effects of synthetic long-chain polyphosphate on blood coagulation and platelet aggregation. Methods: The effect of artificial synthetic long chain poly phosphate on blood coagulation and platelet aggregation was detected by coagulation tests, coagulation factor activity detection and platelet aggregation test,and its mechanism was explored by ELISA, flow cytometry and high content imaging system. Results: The long chain polyphosphates prolonged activated partial thromboplastin time,decreased coagulation factor FⅧ,FⅨ, FⅪ and FⅫactivity, blocked ADP-induced platelet aggregation, and decreased the concentration of calcium and TXA2 in platelet.Conclusion: The synthetic long-chain polyphosphate can inhibit endogenous coagulation and inhibit platelet aggregation,which may be related with the inhibition of intracellular calcium and TXA2.更多还原