【摘要】 通过冷冻干燥技术制备负载辛伐他汀(Simvastatin,SIM)的氧化石墨烯/丝素蛋白(Graphene oxide/silk fibroin,GO/SF)复合屏障膜,并研究其体外细胞相容性及其在引导性骨组织再生中的可应用性。采用扫描电镜、X-射线衍射、紫外分光光度计检测GO/SF屏障膜的形貌、凝聚态结构以及释药性能;激光共聚焦显微镜、CCK-8实验评价小鼠胚胎成骨细胞前体细胞(MC3T3-E1)在屏障膜上的粘附与增殖情况;将GO/SF/SIM膜组、GO/SF膜组、SF/SIM膜组以及SF膜组分别覆盖于SD大鼠颅骨缺损处,术后12周取材分析各组新骨形成情况及GO对大鼠重要脏器的影响。结果显示,GO/SF屏障膜具有表面致密、内部疏松的双层结构,凝聚态结构表现为稳定的Silk II结构;体外释放实验表明该屏障膜具有缓释效果,药物可在15天内被缓慢释放;同时GO/SF屏障膜可很好的支持MC3T3-E1细胞的粘附、生长与增殖,负载SIM后的材料表现出更好的细胞相容性;体内实验结果表明GO/SF/SIM膜组具有最优的骨缺损修复效果,局部应用GO短期观察对大鼠重要脏器(肝、脾、肾)无损害。更多还原

【Abstract】 A graphene oxide/silk fibroin( GO/SF) barrier membrane loaded with simvastatin( SIM) was prepared by a freezedrying method. The biocompatibility of the barrier membrane in vitro and its applicability in guided bone regeneration were investigated. The morphology,structure and the drug release behavior of the membrane were characterized by SEM,XRD and UV-spectroscopy. Laser confocal microscopy and CCK-8 tests were used to evaluate the attachment and proliferation of M C3 T3-E1 dermal cells. Defective skulls of Sprague Daw ley rats were covered with four membranes( GO/SF/SIM membrane,GO/SF membrane,SF/SIM membrane and SF membrane) and their effectiveness for bone regeneration was evaluated. Results indicated that the GO/SF/SIM membrane had a double-layer( dense surface and porous inside) and stable silk II structure. SIM was continuously released from the barrier membrane over 15 days. The GO/SF membrane supported the attachment,grow th and proliferation of M C3 T3-E1 cells. The GO/SF/SIM exhibited the best bone repairing ability among the four membranes and a better biocompatibility in vitro than the GO/SF. The local application of GO had no damage to important organs( liver,spleen and kidney) of the rats in the shortterm observation.更多还原