【摘要】 目的对法医检验常用STR基因座的三带型模式作观察与分析,探讨其群体现象和特点。方法基于STRtyper-21G试剂盒20个STR基因座分析37 737例无关个体的STR分型数据,查找三带型,用不同试剂盒予以验证后,统计其频率并分析其特点。结果 26例疑似三带型中,证实21例,排除2例(为跨基因座稀有等位基因),不能定论3例。三带型的总检出频率为0.0556%(21/37737),其中,FGA、TPOX基因座检出率最高,二者均为0.0106%(4/37737),其次为Penta E为0.0079%(3/37737);Type1三带型为71.4%(15/21),Type2三带型为28.6%(6/21);位于蛋白质编码区的基因座更易发生三带型。结论三带型结果在人群中存在一定比例,判读须慎重。更多还原

【Abstract】 Objective To explore the phenomenon and characteristics of triallelic patterns discovered in the STR loci commonly used for routine forensic practice. Methods Triallelic patterns were screened from 37,737 unrelated healthy individuals’ STR-genotyping data obtained with STRtyper-21 G kit. All the triallelic patterns were confirmed by other different kits. The frequency of triallelic patterns was calculated and their features were analyzed. Results Of the 26 suspected triallelic patterns, 21 ones were confirmed, 3 ones elusive, and the left 2 ones were excluded for them to be the rare alleles spanning across the neighboring locus. The total frequency of triallelic patterns was 0.0556%(21/37737) in autosomal STRs, with 0.0106%(4/37737) at FGA and/or TPOX, plus 0.0079%(3/37737) at Penta E. Of all the triallelic patterns, Type 1 accounts for 71.4%(15/21) and Type 2 for 28.6%(6/21). Conclusion Triallelic patterns are present of certain percentage in population, thus careful judgment is needed in routine forensic work.更多还原