【摘要】 目的研究海洛因对腹腔巨噬细胞分泌白细胞介素-1的影响。方法体外培养腹腔巨噬细胞,用胸腺细胞增殖法检测白细胞介素-1的活性。结果海洛因处理后腹腔巨噬细胞分泌白细胞介素-1增强,通过紫外分光计检测λ=405nm处海洛因处理组A值为700±82.82,而空白对照组的A值为535.6±24.66(P<0.05)。结论海洛因可以通过影响白细胞介素-1进一步影响机体的免疫功能。更多还原

【Abstract】 Objective To study the influence of heroin on the peritoneal macrophages to secrete interleukin(IL)-1. Methods Mouse peritoneal macrophages were cultured in vitro with addition of heroin into the routine medium and measured of the IL-1 activity via thymocyte proliferation method. Results After treated with heroin, the mouse peritoneal macrophages secreted IL-1 increasingly. Through measurement by an ultraviolet spectrophotometer at the λ=405 nm, an A value was obtained about 700±82.82 of the IL-1 activity in the heroin-treated group compared with 535.6±24.66 in the control group(p< 0.05). Conclusion heroin could modulated the mouse immunologic function by promoting IL-1 secretion.更多还原