节点文献

基于ITS和ISSR的羊肚菌种质资源遗传多样性分析

Identification and Phylogenetic of 18 Morchella Strains Based on ITS Sequence and ISSR Analysis

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 杨燕田鸿张小平毋校辉罗金洲

【Author】 YANG Yan;TIAN Hong;ZHANG Xiao-ping;WU Xiao-hui;LUO Jin-zhou;College of Resource,Sichuan Agricultural University;Sichuan Mushiroom Agricultural Development,Co.Ltd.;

【通讯作者】 田鸿;

【机构】 四川农业大学资源学院四川菌之味农业科技有限公司

【摘要】 【目的】目前羊肚菌栽培菌种品性退化,羊肚菌种质资源的筛选评价对于选育优质新品质具有重要意义。【方法】本研究采用核糖体DNA转录间隔区(Internal Transcribed Spacer,ITS)序列分析技术,对收集到的18个羊肚菌菌株进行菌株鉴定和系统发育分析,同时应用ISSR(inter-simple sequence repeat)分子标记手段,进行遗传多样性以及亲缘关系分析。【结果】10个菌株为梯棱羊肚菌(Morchella importuna),2个为六妹羊肚菌(Morchella sextelata),4个为高羊肚菌(Morchella elata),2个野生羊肚菌为粗柄羊肚菌(Morchella crassipes)。ISSR聚类分析显示出供试菌株的总体相似性为0.52,相似系数为0.54时聚为两大类群。【结论】18株菌株间具有明显的遗传多样性。

【Abstract】 【Objective】Presently,due to the degradation of the quality and agronomic traits of cultivation Morchella strains,screening and evaluating of Morchella strain have a great significance for breeding high quality varieties.【Method】ITS sequence analysis was applied to identify the 18 Morchella strains,and ISSR analysis was used to explore the genetic diversity of the 18 Morchella samples.【Result】10 strains were identified as Morchella importuna,2 strains were identified as Morchella sextelata,4 strains were identified as Morchella elata,and 2 strains were identified as Morchella crassipes.The cluster map showed that the overall similarity of tested strains was 0.52,and the tested strains were clustered into two groups at similarity coefficient of 0.54.【Conclusion】Morchella strains have a distinct genetic diversity.

【关键词】 羊肚菌ITSISSR系统发育
【Key words】 MorchellaITSISSRPhylogenetic
【基金】 四川省“十三五”育种攻关项目(2016NYZ0040)
  • 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2018年10期
  • 【分类号】S646.7
  • 【被引频次】15
  • 【下载频次】431
节点文献中: 

本文链接的文献网络图示:

本文的引文网络