【摘要】 旨在鉴定和分析肉鸡肌肉和脂肪组织基因组差异剪接基因(differential splicing gene,DSG)。本研究采用Illumina Hiseq 2500测序平台对AA肉鸡胸肌和腹部脂肪组织各3个样品进行转录组测序,使用rMATS软件检测样品中可变剪接事件(alternative splicing,AS)和组织间的DSG,并对DSG进行GO功能富集和KEGG通路分析。结果表明,在肉鸡肌肉和脂肪组织中分别检测到(5 966.00±111.66)和(6 757.00±156.51)个可变剪接事件(P=0.002)。5种可变剪接类型中以外显子跳跃(skipped exon,SE)为主,分别占肌肉和脂肪组织中可变剪接事件总数量的54.92%和52.67%。肌肉和脂肪组织基因组间检测到513个显著的DSGs。GO基因富集分析发现,93个DSGs显著富集于细胞组分和分子功能中。信号通路分析发现,31个DSGs富集在肌动蛋白细胞骨架调节、焦点粘连、丙酮酸代谢和细胞内吞作用信号通路中,其中14个DSGs在肌肉和脂肪组织基因组中的表达水平未发生显著变化。本研究通过对肉鸡肌肉和脂肪组织基因组中DSG的鉴定与分析,为研究可变剪接及其调控机制、组织间差异剪接的多样性提供理论依据。更多还原

【Abstract】 The objective of this study was to identify and analyze the differential splicing gene(DSG)between muscle and fat tissues genome in AA broilers.The transcriptomic profiling of breast muscle and abdominal fat tissues(n=3)in AA broilers were sequenced using Illumina Hiseq 2500 technology.The alternative splicing event(AS)and DSG between muscle and fat tissues genome in AA broilers were identified using rMATS software.The GO function enrichment and KEGG pathway analysis were performed for DSG.The results showed that(5 966.00±111.66)and(6 757.00±156.51)(P=0.002)alternative splicing events(AS)were identified in genome of muscle and fat tissues,respectively in AA broilers.Skipped exon(SE)was the main events with the highest frequency of 54.92% and 52.67% of the total number of AS in muscle and fattissues,respectively;A total of 513 significant DSGs were identified between muscle and fat tissues genome.The GO enrichment analysis indicated that 93 DSGs were significantly enriched in cellular component and molecular function.A total of 31 DSGs were significantly enriched in the regulation of actin cytoskeleton,focal adhesion,pyruvate metabolism and endocytosis pathways;The mRNA expression levels of 14 DSGs were not significantly different between muscle and fat tissues.Through the genome-wide identification and analysis of DSG in muscle and fat in AA broilers,the results of this study will provide insight into the mechanisms of alternative splicing and diversity of differential splicing.更多还原