【摘要】 细胞微环境的物理性质在各种生理和病理调控方面起着非常重要的作用,其中,微环境表面形貌对细胞粘附、形态和迁移有明显影响。借助微纳米加工技术可以加工出不同尺度、不同形貌的类细胞微环境,以对细胞行为进行调控。鉴于SUM159细胞(人类乳腺癌细胞)与MCF10A细胞(人类正常乳腺上皮细胞)是研究乳腺癌方面的重要细胞模型,加工了一批具有不同尺寸的PDMS沟槽结构,研究几何尺寸的改变对SUM159细胞与MCF10A细胞空间分布的影响,分析了这2种细胞在不同几何尺寸下的取向。由于大部分器官和组织是由多细胞组成,研究将这2种细胞进行共培养,并探究了在混合状态下PDMS沟槽基底对其行为的影响。研究结果在癌症转移中有潜在应用。更多还原

【Abstract】 The physical properties of the cellular microenvironment play a key role in various aspects of physiological and pathological regulation,among them,the surface morphology of the microenvironment has significant effects on cell adhesion,morphology and migration.With the aid of micro-nanofabrication technology,the extracellular-like environmental with different scales and shapes can be fabricated to regulate the cell behaviors.Considering that SUM159 cells(human breast cancer cells)and MCF10 Acells(human normal breast epithelial cells)are important cell models for the study of breast cancer,a batch of PDMS groove structures with different scales were fabricated to study the effects of geometric changes to SUM159 cells and MCF10 Aspatial distribution,and it was also analyzed on the orientation of the cell body with different geometric scales.Since most organs and tissues are composed of multiple cells,the co-culture of the two cells was used to investigate the effects of cellular behavior by PDMS groove substrate under mixing conditions.The results of the study had potential applications in cancer metastasis.更多还原