At the early stage of apoptosis, phosphatidylserine(PS) will turn over from the inner cell membrane to the outer cell membrane. When calcium ions are present, Annexin A5 can combine to PS at high affinity and this is the principle of apoptosis detection. Apoptosis detection based on Annexin A5 labeled with green fluorescent probes/iodide by FCM(flow cytometry) is sensitive, efficient and specific. Annexin A5-EGFP fusion protein is a simple, reliable apoptosis detection probe and it is easy to prepare. Yello...
The Influence of Structrue of Yellow Fluorescent Proteins on theApoptotic Detection Ability of Annexin A5Gao Mengyue1, Wang Yunke1, Huang Xianjie1, Zhang Jing1*, Hua Zichun1,2,3*(1School of Life Sciences, the State Key Laboratory of Pharmaceutical Biotech