【摘要】 重复序列是基因组的重要组成部分,对生物的进化、遗传和基因的表达调控有重要作用.本研究利用生物信息学方法,在异常汉逊酵母菌株792全基因组de novo测序的基础上,对基因组重复序列的分布特征进行了研究.结果表明,其全基因组中重复序列总长346 417bp,其中,串联重复序列238 164bp,散在重复序列108 253bp,占基因组长度的2.52%,平均每4.05Kb就有一个重复序列.微卫星DNA序列中重复单元拷贝数大多低于15次,其优势碱基类型为三核苷酸重复,重复单元基序为AAC的微卫星序列数目最多;小卫星DNA序列重复单元拷贝数小于微卫星DNA,主要分布1~3次,重复单元大于15bp的小卫星序列数目随着重复单位长度的增加呈下降趋势.散在重复序列中长末端重复序列(LTR)数目最多,滚环(RC)平均长度最长.本研究结果为汉逊酵母菌的分子定向育种和SSR分子标记的开发提供理论基础.更多还原

【Abstract】 Repeat sequence is an important part of the genome and plays an important role in the evolution of organisms,genetics,and regulation of gene expression.Here,bioinformatics methods were used to study the distribution characteristics of repeat sequences in the genome of Hansenula anomala 792 based on de novo sequencing.The results showed that the total length of the repeat sequences in the whole genome was 346 417 bp,in which the tandem repeats were 238 264 bp,interspersed repeats were 108 253 bp,accounting for 2.52% of the total genome length,and there was one repeat sequence per 4.05 Kb.The copy number of repeat units in microsatellite DNA sequences was mostly less than 15 times,the dominant base type was trinucleotide repeats,and the largest number of repeat unit motif was AAC;the copy number of repeat units in minisatellite DNA was less than that in microsatellite DNA and it mainly distributed from 1 to 3 times,the number of minisatellite DNA with repeat unit greater than 15 bp is decreasing with the increase of repeat unit length.The number of long-terminal repeats(LTRs)was the largest in interspersed repeats,and the average length of rolling coils(RCs)was the longest.Our results will provide a theoretical basis for the molecular breeding and developing of SSR molecular markers of Hansenula anomala.更多还原