【摘要】 以牦牛骨为原料,酶解法制备可溶性骨胶原多肽,考察蛋白酶种类、底物质量浓度、酶底比、酶解时间、pH、酶解温度对酶解液水解度和多肽得率的影响,在单因素试验基础上以多肽得率为指标进行响应面优化实验,最终确定碱性蛋白酶为试验最适用酶,最佳酶解条件为:底物质量浓度3.5 g/d L,酶底比4 000 U/g,pH 7.0,酶解时间4 h,此时多肽得率为22.06%。对酶解产物进行加工特性测定表明其具有良好的溶解性和稳定性。更多还原

【Abstract】 Yak bone protein was hydrolyzed with protease for the preparation of soluble polypeptides. Alkaline protease was proved to be the optimal activator and the single-factor method was used to analyze the effect of time,pH,substrate concentration,enzyme/substrate ratio and temperature on the degree of hydrolysis and yield of polypeptides. Factors such as substrate concentration,enzyme/substrate ratio,hydrolysis pH and time were screened out for response surface analysis based on polypeptides yield. On the optimal hydrolysis conditions of substrate concentration3.5 g/d L,enzyme/substrate ratio 4 000 U/g,hydrolysis pH 7.0 and time 4 h,the actual value of yield was 22.06% and the peptides were detected with good solubility and stability.更多还原