【摘要】 目的以铜绿微囊藻FACHB-905(Microcystis aeruginosaus FACHB-905)为实验对象,研究和比较乳酸、洋葱水浸提液以及洋葱乳酸浸提液的抑藻效应,探讨洋葱乳酸浸提液对细胞内外藻毒素和多糖的影响。方法分别采用1.25、2.5、5、10和15μL/L的乳酸、洋葱水浸提液以及洋葱乳酸浸提液作用于铜绿微囊藻,测定藻密度变化;采用酶联免疫分析方法测定抑藻效果最佳的浸提液对藻细胞内外藻毒素和多糖变化的影响。结果单独乳酸和洋葱水浸提液对铜绿微囊藻的抑制率在最大浓度96 h时仅达到71.72%和75.17%;而洋葱乳酸浸提液处理48 h其2.5μL/L实验组的抑制率就达到了78.87%,96 h时达到90.34%,15μL/L组96 h时则达到了99.59%。洋葱乳酸浸提液作用于铜绿微囊藻96 h时,藻细胞内外微囊藻毒素和大分子多糖的含量均随着浸提液浓度增大而增加。结论洋葱乳酸浸提液对铜绿微囊藻的生长有很强的抑制作用,对藻细胞内外微囊藻毒素和多糖含量有升高作用。更多还原

【Abstract】 Objective Microcystis aeruginosa FACHB-905 was used as the experimental material to study the inhibitory effect of lactic acid,water leaching solution of Allium cepa L. and lactic acid leaching solution of A. cepa L. and the influence of the lactic acid leaching solution of A. cepa L. on microcystins and polysaccharides of the M.aeruginosa. Methods The concentration of 1. 25,2. 5,5,10 and 15 μL/L of the each solution was used to stress the M. aeruginosa,and the changes of the algal density was assayed,the microcystins and polysaccharides contents in the inside and outside of M.aeruginosa were tested using enzyme-linked immunosorbent assay for the group with the best inhibitory effects. Results The inhibitory ratios of lactic acid alone and water leaching solution of A. cepa L. reached 71. 72% and 75. 17% at the maximum concentration of 96 h,while the 2. 5 μL/L of lactic acid leaching solution of A. cepa L.reached 78. 87% at 48 h. At 96 h,it reached 90. 34%,and the maximum concentration reached 99. 59% at 96 h. The concents of microcystins and polysaccharides increased withthe increase of concentration of lactic acid leaching solution of A. cepa L. in 96 th hours.Conclusion The inhibitory effect of lactic acid and water leaching solution of A. cepa L.to M. aeruginosa was less than lactic acid leaching solution of A. cepa L.,and lactic acid leaching solution of A. cepa L. had the strongest inhibitory effect on the growth of M.aeruginosa. The contents of microcystin and polysaccharide of M. aeruginosa in or out of the algal cells were also affected.更多还原