【目的】通过对南海Ⅰ号沉船出水木质文物标本中细菌类群的分析,了解饱水木质文物中的细菌类群并推测细菌对木质文物损害的机制。【方法】应用Illumina Mi Seq测序平台对采自该沉船的10份木质文物标本中细菌V3–V4序列进行测序与分析,比较各标本中细菌群落的组成差异。【结果】根据97%序列相似性得到3 780不同的细菌OTUs,分属34目、35科的187个属;多数细菌OTU属于变形菌门(Proteobacteria)细菌,占全部细菌OTU的52.9%,在细菌纲水平上γ-变形菌纲(γ-Proteobacteria)(占17.9%)是丰度最高的细菌纲。德沃氏菌属(Devosia)(3.5%)是南海一号沉船样品丰度最高的属,其他分别属于甲基娇养杆菌属(Methylotenera)(2.4%)、鼠尾菌属(Muricauda)(1.2%)。其中氢噬胞菌属(Hydrogenophaga)、中国农大湖积物杆菌(Lacibacter cauensis)、德氏食酸菌(Acidovorax delafieldii)、德沃斯氏菌属(Devosia)、沉积物杆状菌属(Sediminibacterium)、缺陷短孢单胞菌(Brevu...
【英文摘要】
[Objective] To understand the distribution of microorganisms and their effects on the waterlogged wooden relics collected from the Nanhai No. 1 shipwreck. [Methods] Prokaryotic microorganisms in 10 wooden relics samples collected from the Nanhai No. 1 shipwreck were identified by using high throughput sequencing technique. The composition and diversity of the bacterial communities in each sample were analyzed. [Results]Total 121 394 313 bacterial sequences with an average reading length of 458 bp were obtai...
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