[Background] Misgurin is an important component of nonspecific immune defense system in Misgurnus anguillicaudatus. It has broad spectrum and strong antimicrobial ability. Therefore, it is necessary to obtain large quantities of antimicrobial peptides. [Objective] To achieve high-efficient expression of misgurin. [Methods] Misgurin gene was cloned into expression vector p PIC9 K in order to construct recombinant expression plasmid p PIC9 K-misgurin. Using Sal I restriction endonuclease digest linearization ...
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