【摘要】 目的从系统生物学的角度探讨miRNA与乳腺癌的发病机制的关系。方法应用转录组测序技术分析人正常乳腺上皮细胞(HMEC)及乳腺癌细胞(MCF7)的转录组,建立差异表达miRNA和差异表达基因的调控关系,对差异表达miRNA调控的基因进行通路富集分析。结果发现456个差异表达miRNA,其中上调表达217个,下调表达239个;发现4 566个差异表达基因,其中上调表达2 710,下调表达1 856;建立了包含16 554个miRNA对靶基因的调控关系网络;获得由hub基因和hub miRNA构成的乳腺癌基因调控核心网络;被调控的差异基因富集于64条通路上。结论 hsa-miR-19a-3p与hsa-miR-30c-5p等hub miRNA靶向CBX5和POU2F等hub基因与乳腺癌的生成密切相关;PI3K-Akt与细胞基因粘连等通路在乳腺癌的基因调控机制中起着重要作用。更多还原

【Abstract】 Objective To explore the relationship between miRNA and pathogenesy of breast cancer in terms of systems biology. Methods NGS was performed to analyze the transcriptome of HMEC and MCF7. The regulatory relationship between differently expressed genes(DEGs) and miRNAs was established. DEGs regulated by miRNA were identified with pathway enrichment analysis. Results There were a total of 456 differently expressed miRNA, including 217 up-regulated miRNAs and 239 downregulated miRNAs. There were a total of 4 566 DEGs, including 2 710 up-regulated genes and 1 856 down-regulated genes. 16 554 regulatory relations between miRNAs and target genes were established. Core regulation network in breast cancer including hub genes and hub miRNAswere established. DEGs regulated by miRNA were enriched in 64 pathways.Conclusion Target relationship between hub miRNAs such as hsa-miR-19 a-3 p and hsa-miR-30 c-5 p and hub genes such as CBX5 and POU2 F is closely related to the generation of breast cancer; pathway such as PI3 K-Akt and focal adhesion play an important role in gene regulation mechanism of breast cancer.更多还原