【摘要】 目的:同步记录大鼠背外侧前额叶(DLPFC)和杏仁核(amygdala)细胞外电活动和外周生理指标,分析神经网络振荡和探究伤害性刺激的影响。方法:取成年SD大鼠12只,同步记录DLPFC亚区岛叶无颗粒皮质背部(AID)和杏仁核亚核基底外侧杏仁核(BLA)的细胞外电活动、心电和呼吸肌肌电,分析神经网络振荡并观察夹尾刺激后各项指标的变化。结果:①夹尾刺激时AID和BLA的放电频率可逆性升高(P<0.05);②夹尾刺激时AID和BLA的低频振荡(delta,theta)能量减弱(P<0.05),高频振荡(gamma,fast)能量仅有增强趋势(P> 0.05),且都在刺激后恢复;③夹尾刺激后两核团间delta、theta、fast振荡的相位锁值都比刺激前降低(P<0.05);④夹尾刺激加快了心率(P<0.05)、呼吸频率(P<0.01),刺激后呼吸频率逐渐恢复。结论:夹尾刺激能使AID和BLA的兴奋性增强,但使两核团间的相位同步程度减弱,这可能与伤害性刺激引起的负性情绪有关。更多还原

【Abstract】 Objective: To simultaneously record the extracellular electrical activities and peripheral physiological indexes in the dorsolateral prefrontal cor-tex( DLPFC) and amygdala of rats for observing the effects of nociceptive stimulation on neural network oscillation.Methods: Indexes were simultaneouslymaintained on the extracellular electrical activities in the dorsal agranular insular area( AID) in DLPFC and basolateral amygdala( BLA) in amygdala inadult SD rats( n = 12) as well as their electrocardiogram and electromyography of respiratory muscles following tail pinch-induced stimulus,and the stimula-tion on neural network oscillation was observed and analyzed.Results: ①The discharge rate was reversibly increased in AID and BLA during the tail pinchstimulation( P<0.05); ②The tail pinch stimulation reduced the power at delta and theta rhythm( P<0.05),whereas increased the power at gamma and fastrhythm to some extent( P>0.05),and the power changes were all reversible; ③The phase locking values were significantly reduced after the stimulation atthe delta,theta and fast rhythm compared to pre-stimulation values( P<0.05); ④The tail pinch stimulation not only increased the heart rate( P<0.05),but also reversibly increased the respiratory frequency( P<0.01).Conclusion: Tail pinch-induced stimulus may increase the excitability in AID and BLA,yet weaken strength of phase coupling between AID and BLA,which may be related to negative emotion caused by nociceptive stimulation.更多还原