【摘要】 目的探究SMP30对肝癌细胞的调控作用,分析SMP30沉默对人肝脏细胞系Hep G2增殖、凋亡、侵袭等作用的影响。方法培养Hep G2细胞株,从第二代开始隔一代收集培养细胞,进行细胞总蛋白的提取,提取肝癌细胞的细胞核蛋白、细胞浆蛋白、细胞膜蛋白,利用蛋白质印迹法检测SMP30蛋白含量,将肝癌细胞注入裸鼠腋部皮下作为裸鼠种植模型,将其分为2周组、4周组、6周组,每组各10只,选择10只无荷瘤裸鼠作为对照组,对裸鼠血清进行Western blot检测,分析SMP30m RNA的相对表达量。结果与结论肝癌发展状况与SMP30表达水平变化密切相关,DNA甲基化能够降低肝癌SMP30蛋白,SMP30可以有效抑制肝癌细胞的侵袭能力。更多还原

【Abstract】 Objective to investigate regulation effect of SMP30 on hepatoma cells,analyze effect of SMP30 silence on proliferation,apoptosis and invasion of human liver cell line Hep G2.Methods culture Hep G2 cell line,collecte cultured cells from second generations every other generation,abstract cell protein including cell nuclear protein,cytoplasmic protein and cell membrane protein,detect SMP30 protein content with Western blotting method,inject hepatoma cells into nude mice armpit subcutaneous area as nude mouse planting model.Divide them into 2-week group,4-week group and 6-week group,10 cases in each,choose 10 nude mice without tumor bearing as control group.Detect serum of nude mice by Western blotting,analyze relative expression of SMP30 m RNA.Conclusion liver cancer development is closely related to SMP30 expression level changes.DNA methylation can reduce SMP30 protein in liver cancer,and SMP30 can inhibit the invasion ability of hepatoma cells effectively.更多还原