【摘要】 优化金线莲组培条件的试验结果表明:以75%乙醇和0.1%HgCl浸泡处理8 min的方式对金线莲外植体进行消毒,可以取得较好的处理效果;以MS为基本培养基并添加激素6-BA/2 mg·L^-1和NAA/0.5 mg·L^-1以增殖继代培养基的处理效果最优;在培养基中添加20%香蕉对于芽苗的增殖及壮苗的培养均有极大的促进作用;液态培养基显著优于半液态和固态培养基;原球茎增殖途径增殖倍数高且增殖速度快,但培养出来的芽苗的质量相对较弱。更多还原

【Abstract】 The experiment which was carried out to optimize the conditions for the tissue culture for Anoectochilus roxburghii showed the following results: firstly, it achieved good effects to disinfect the explants of Anoectochilus roxburghii by soaking them in 75% ethyl alcohol and 0.1%HgCl solutions for 8 minutes; secondly, the effect of proliferating the subculture medias was optimized by use of minimal medium combined with hormone 6-BA/2 mg·L^-1 and NAA/0.5 mg·L^-1; thirdly, it was greatly conducive to the bud seedling proliferation and the strong seedling development to add 20% banana pulp to the media; fourthly, the liquid culture medium were significantly better than semi-liquid and Solid mediums; fifthly, the proliferation multiple and speed improved by the way of protocorm proliferation, while the cultured bud seedlings were relatively weak.更多还原