【摘要】 [目的]该实验制备通窍散瘀方微乳,并对其含量、理化性质、刺激性进行评价。[方法]实验以葛根素溶解度为指标,筛选出合适的乳化剂、助乳化剂、油相。再通过伪三元相图,优化微乳处方。[结果]得到制剂处方为EL35,2丙二醇-IPM-水,四者比例为29.23∶29.23∶5.18∶36.34(W∶W∶W∶W)。得到的O/W型微乳,外观澄清透明,PH均值约为5.31。透射电镜下观察,圆整、均匀。激光粒径测定仪测定平均粒径约为17.86 nm,Zeta电位约为-4.90。葛根素载药量约为:68.64 mg/g,芍药苷载药量约为26.96 mg/g。通过大鼠鼻中隔切片,研究通窍散瘀微乳和通窍散瘀溶液对鼻黏膜的刺激性、损伤性作用及可逆性。[结论]结果显示通窍散瘀微乳能显著提高难溶性药物葛根素的载药量,并且形成均一稳定的体系。可为通窍散瘀方给药提供新的剂型。微乳的刺激性小于溶液,且微乳损伤是可逆的。更多还原

【Abstract】 [Objective] The experiment is to prepare Tongqiao Sanyu microemulsion, and investigate its content,physical,chemical properties and irritation. [Methods] Use the solubility of puerarin as an index, investigate the appropriate emulsifier, co-emulsifier and oil phase. And then through the pseudo ternary phase diagram, optimize the microemulsion prescription. [Results] The formulation is EL35-1, 2-propanediol-IPM-water, and the ratio is 29.23∶29.23∶5.18∶36.34(W∶W∶W∶W). System of Tongqiao Sanyu microemulsion for the O/W microemulsion, the appearance of clear and transparent, The p H is 5.31. Placed under the transmission electron microscope observation, round,uniform.. The average particle size was 17.86 nm and the zeta potential was-4.90 by laser particle size analyzer. The dose of Puerarin was 68.64 mg/g, and paeoniflorin was 26.96 mg/g.Toad palate cilia irritation test results show that the drug suspension and microemulsion preparations are certain irritation. [Conclusion] Results show that Tongqiao Sanyu microemulsion can significantly improve the insoluble drug puerarin drug loading, and the formation of uniform and stable system. Tongqiao Sanyu microemulsion can provide a new dosage form for Tongqiao Shuyu recipe.The irritation of microemulsion is less than that of the solution, and the damage of microemulsion is reversible更多还原