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不同自生固氮菌对盐碱地玉米生长与土壤养分特性的影响

Effects of Nitrogen Fixing Bacteria on Growth of Maize and Nutrient Characteristics of Coastal Saline Soil

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【作者】 王慧桥陈为峰宋付朋董元杰

【Author】 WANG Hui-qiao;CHEN Wei-feng;SONG Fu-peng;DONG Yuan-jie;College of Resources and Environment, Shandong Agricultural University, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer;

【通讯作者】 董元杰;

【机构】 山东农业大学资源与环境学院土肥资源高效利用国家工程实验室

【摘要】 为探讨4种自生固氮菌,包括菌c:争论贪噬菌(Variovorax paradoxus),菌d:玉米固氮螺菌(Azospirillum zeae),菌f:苜蓿中华根瘤菌(Sinorhizobium meliloti)和菌j2:荧光假单胞菌(Pseudomonas fluorescens)对盐碱地玉米生长和土壤养分特性的影响,在田间微区试验条件下,研究了土施4种固氮菌对玉米大口期和成熟期不同器官氮素积累量及成熟期产量与产量构成因素的影响,并探讨了4种菌对土壤有效氮含量和土壤脲酶活性的影响。结果表明:菌剂处理可以增加大口期与成熟期玉米根、茎与叶的干物质质量,提高大口期玉米根冠比;增加玉米籽粒中氮素积累量,其中f与j2处理较CK差异显著;各菌剂处理玉米产量较CK分别增加了18.09%、77.91%、157.0%和108.1%,菌f和j2差异显著。不同菌剂处理均提高了土壤有效氮、速效磷和速效钾含量,在成熟期,d处理较CK土壤有效氮含量显著增加了24.32%。在大口期与成熟期,各菌剂处理均提高了土壤脲酶活性。综上,各菌剂处理能提高土壤有效氮含量,提高玉米产量。本研究可为研制滨海盐渍土专用微生物菌肥提供理论依据及技术支撑。

【Abstract】 In order to explore the effects of saline soil inoculated with azotobacter on soil nutrient characteristics and growth of maize, four azotobacter isolates, including c(Variovorax paradoxus), d(Azospirillum zeae), f(Sinorhizobium meliloti) and j2(Pseudomonas fluorescens), were inoculated to soil in micro plot and nitrogen accumulation, yield and its components of maize, soil available nutrient and urease were examined at the bellmouthed and ripening stages of maize. Compared with control, the inoculated treatment increased the dry matter weight of maize roots, stems and leaves at bellmouthed and mature stages, and root to shoot ratio of maize at bellmouthed stage. Compared with control,the maize yield was increased by 18.09%, 77.91%, 157.0% and 108.1%, respectively and its difference between f and j2 was significant. The contents of available nitrogen, available phosphorus and available potassium in soil were increased in different inoculant treatments. In the mature stage, the available nitrogen content of d strain was significantly increased by 24.32% compared with CK. During bellmouthed and mature stages, the soil urease activity were increased in the inoculant treatment. In conclusion, the treatment of microbial inoculants could effectively improve the content of soil available nitrogen and increase the yield of maize. This results could provide theoretical basis and technical support for the development of special microbial fertilizer in coastal saline soil.

【关键词】 固氮菌滨海盐渍土玉米生长土壤脲酶
【Key words】 AzotobacterSaline soilMaizeGrowthSoil urease
【基金】 山东省重点研发计划(2016CYJS05A02);山东省重大科技创新工程项目(2017CXGC0301);国家重点研发计划(2017YFD0201705);国家重点基础研究发展计划(973计划)项目(2015CB150404)资助
  • 【文献出处】 土壤通报 ,Chinese Journal of Soil Science , 编辑部邮箱 ,2018年06期
  • 【分类号】S513;S158
  • 【被引频次】16
  • 【下载频次】454
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