Effect of Combined Prescription and Separate Prescription of Huayu Tongluo Traditional Chinese Medicine on Blood Correlation Index and the Expression of ZO-1 in Renal Podocytes of DN Rats
通过化瘀通络中药全方及拆方对糖尿病肾病(DN)大鼠的24 h尿蛋白定量(UTP)、血脂、血小板活化指标及肾脏足细胞ZO-1的调节作用,探讨化瘀通络中药全方、拆方的不同作用特点及防治DN的可能作用机制。将健康雄性SD大鼠60只分为正常组(C组)10只,造模组50只。造模组大鼠选择高脂高糖饲料喂养联合腹腔注射链脲佐菌素(STZ,1%)35 mg/kg复制糖尿病(DM)大鼠模型,72 h后检测血糖,将成模大鼠(血糖≥16.7mmol/L)随机分为模型组(M组)、化瘀通络中药全方组(Q组)、通络中药组(T组)、化瘀中药组(H组)。按人/鼠体表面积比率换算等效计量法计算各组大鼠用药量,同时C、M两组大鼠灌以等量饮用水,1次/日,连续16周,分别检测各组大鼠24 h UTP、血脂、血小板活化指标等;采用实时荧光定量(Real-time PCR)、蛋白印迹(Western-Blot)方法检测各组大鼠肾组织足细胞ZO-1的基因及蛋白表达情况。实验结果,与C组比较,其余各组大鼠血糖、24 h UTP明显升高(P<0.01),血脂、血小板活化指标出现明显变化(P<0.05,P<0.01);与M组相比,各中药组24 h UTP均明...
【英文摘要】
To investigate the effect of combined prescription and separate prescription of Huayu Tongluo traditional Chinese medicine upon 24 h urine protein quantitation,blood lipid,platelet activation index and the expression of ZO-1 in podocytes of rats with diabetic nephropathy. Totally 60 male sprague-dawley rats were randomly selected as the normal control group( C group,n = 10),the other 50 rats were injected with 1% streptozotocin( 35 mg/kg) after feeding high fat and high glucose diet for one month. The diabe...
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