【摘要】 制备了水溶性的上转换荧光纳米材料,在其表面修饰赭曲霉毒素A(OTA)适配体作为能量供体探针;在金纳米粒子表面修饰OTA适配体互补链作为能量受体探针,构建了OTA适配体传感器。在最优条件下,OTA的检测范围为0.001~10 ng/mL,检出限可达0.001 ng/mL。将其应用于啤酒样品中OTA的检测,当加标水平为0.01、0.1、1.0 ng/mL时,回收率为100%~119%,相对标准偏差为4.3%~4.9%,表明该方法可用于实际样品检测。该方法具有灵敏度高、特异性好、操作简单、成本较低等优点。更多还原

【Abstract】 Upconversion nanoparticles were synthesized and modified with OTA aptamer,and gold nanoparticles were modified with complementary DNA strand( c DNA). Based these nanoparticles,a FRET aptasensor for OTA was successfully fabricated by using upconversion nanoparticles as energy donor and gold nanoparticles as energy acceptor. In the optimum conditions,this aptasensor obtained a detection limit of 0. 001 ng/mL with a linear range of 0. 001-10 ng/mL. The above method was applied in the detection of OTA in beer samples. At spiked levels of 0. 01,0. 1,1. 0 ng/mL,the recoveries for OTA ranged from 100% to 119% with the relative standard deviations( RSDs) of4. 3%-4. 9%. This method also demonstrated its advantages such as high sensitivity,good specificity,simplicity and low cost.更多还原