【摘要】 目的:利用基因芯片检测技术检测幽门螺旋杆菌(Hp)对抗菌药物的耐药性,分析深圳地区Hp耐药状况,为该地区临床根除Hp合理选择抗菌药物提供依据。方法:采用基因芯片检测技术对2015年2月至2017年2月北京大学深圳医院收治的244例合并Hp感染患者的胃黏膜组织进行克拉霉素、左氧氟沙星的耐药性检测,观察不同性别、不同年龄段患者对克拉霉素、左氧氟沙星的耐药情况。结果:共纳入感染Hp的患者244例(男性141例,女性103例),对克拉霉素耐药者有56例,耐药率为22.9%,对左氧氟沙星耐药者有13人,耐药率为5.3%,对克拉霉素、左氧氟沙星双重耐药者有5人,耐药率为2.0%,男女性患者对克拉霉素耐药率比较,差异无统计学意义(P>0.05)。按年龄段分组的耐药率显示,30岁以上年龄组的抗菌药物耐药率逐渐增加(17.6%、24.3%、39.1%),60岁以后年龄组的耐药率则呈下降趋势(13.5%、2.7%)。结论:深圳地区对克拉霉素的耐药性较高,与国内平均水平相近,对左氧氟沙星的耐药性较低,明显低于国内平均水平,本研究为该地区合理选用抗菌药物根除Hp提供了理论依据。更多还原

【Abstract】 Objective To detect the drug resistance of Helicobacter Pylori(H. pylori) by gene chips and analyze the status of antibiotics resistance of H. pylori in Shenzhen District of Guangdong. In order to provide a theoretical basis for reasonable clinical treatment of H. pylori eradication in this area. Method The detection of clarithromycin and levofloxacin in gastric mucosal tissues of 244 patients in Peking university Shenzhen hospital from February 2015 to February 2017 with Hp infection was performed using gene chip detection technology. The drug resistance of clarithromycin and levofloxacin in patients of different genders and ages were observed. Result A total of 244 patients(141 males and 103 females) infected with Hp were included. 56 patients were resistant to clarithromycin, the drug resistance rate was 22.9%, and 13 patients were resistant to levofloxacin. The drug rate was 5.3%. There were 5 people who had double resistance to clarithromycin and levofloxacin, and the drug resistance rate was 2.0%. There was no significant difference between male and female patients in the resistance rate to clarithromycin(P>0.05). The rate of drug resistance by age group showed that the rate of antibiotic resistance in the 30~+ age group gradually increased(17.6%, 24.3% and 39.1%), and the resistance rate in the age group after the age of 60 showed a downward trend(13.5 %, 2.7 %). Conclusion The resistance to clarithromycin in Shenzhen, Guangdong is relatively high. It is similar to the domestic average, and its resistance to levofloxacin is low, which is significantly lower than the domestic average level. This study provides a theoretical basis for the rational use of antibiotics to eradicate Hp in the region.更多还原