【摘要】 目的探讨大蒜素与5-FU联合应用治疗结肠癌的作用机制。方法利用HT-29细胞模拟结肠癌,设置空白对照组、大蒜素组(15μg/m L)、5-FU组(15μg/m L)和大蒜素+5-FU组(各15μg/m L)共4组,采用CCK-8法测定细胞增殖抑制率,流式细胞术观察细胞凋亡情况,Real-time PCR检测m TOR、PTEN、Bcl-2与Bax mRNA表达情况。结果CCK-8检测结果显示,与空白对照组比较,各用药组均可以抑制HT-29细胞的增殖(P<0.05)。其中大蒜素+5-FU组的抑制作用最为显著;流式细胞术检测结果显示,各用药组凋亡细胞显著多于空白对照组,大蒜素+5-FU组凋亡细胞最为显著;Real-time PCR结果显示,与空白对照组比较,各用药组Bcl-2、m TOR的mRNA表达量显著降低而Bax、PTEN的mRNA表达量显著升高,大蒜素+5-FU组变化最为显著。结论大蒜素与5-FU联合应用可以抑制HT29细胞的增殖,促进凋亡,并且大蒜素具有增强5-FU诱导结肠癌细胞凋亡的能力。更多还原

【Abstract】 Objective The aim of this study was to explore the mechanism of allicin combined with 5-FU in the treatment for colon cancer. Methods HT-29 cells were divided into 4 groups as control,allicin( 15 μg/m L),5-FU( 15 μg/m L) and allicin( 15 μg/m L) combined with 5-FU( 15 μg/m L) groups. The inhibitory rate of cell proliferation was determined in HT-29 cells by CCK-8 assay. Flow cytometry was used to observation of apoptosis. The levels of m TOR,PTEN,Bcl-2 and Bax mRNA expression were determined by Real-time PCR. Results Compared with the blank control group,proliferation of HT-29 cells was inhibited in the drug or allicin groups( P < 0. 05). Among them,the allicin-associated 5-FU group had the most significant inhibitory effect. The apoptotic cells in each drug group were significantly more than the blank control group,and the apoptotic cells in the allicin combined with 5-FU group were the most significant. Compared with the blank control group,the levels of Bcl-2 and m TOR mRNA significantly decreased and the expression levels of Bax and PTEN mRNA increased significantly in each drug group. The most significant changes were observed in the allicin and 5-FU combination group. Conclusion The combination of allicin and 5-FU could inhibit the proliferation and promote apoptosis in HT29 cells. Allicin has the ability to enhance 5-FU-induced apoptosis in colon cancer cells.更多还原