【摘要】 以黑农44(耐旱型品种)和黑农65(敏感型品种)为材料,采用EM-50和称重法相结合控制水分,在初花期设置不同程度(轻度、中度、重度)干旱处理,研究干旱胁迫对大豆叶片渗透调节物质、抗氧化酶活性、抗氧化能力和膜脂过氧化作用的影响。试验结果表明,随着干旱胁迫程度的增强,渗透调节物质脯氨酸和可溶性糖的含量呈升高趋势,可溶性蛋白含量先升高后降低;抗氧化酶活性呈现先升高后降低趋势,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性的最大值出现在中度干旱胁迫,而过氧化物酶(POD)活性的最大值则出现在轻度干旱胁迫;超氧阴离子清除能力(SASC)、羟自由基清除能力(HRS)和总抗氧化能力(T-AOC)呈先升高后降低趋势;重度干旱条件下,耐旱型品种的渗透调节物质含量、抗氧化酶活性、抗氧化能力均显著大于敏感型品种。膜脂过氧化作用随干旱胁迫程度增强呈逐渐升高趋势,敏感型品种显著大于耐旱型品种。以上结果表明,黑农44适应和抵御干旱的能力显著大于黑农65。更多还原

【Abstract】 Two varieties, Heinong 44(drought tolerant) and Heinong 65(drought sensitive), were used as experimental materials and treated in different degrees(mild, moderate, severe) of drought at the beginning of flowering stage to study the effects of drought stress on osmotic adjustment substances, antioxidant enzyme activities, antioxidant capacity and membrane lipid peroxidation in soybean leaves at Northeast Agricultural University in a rainproof chamber glass in 2016. Soil moisture was controlled by weighing method and EM-50 measurement. The results showed that the contents of proline and soluble sugar increased with the aggravation of drought stress degree, and the soluble protein contents increased firstly and then decreased. The activities of antioxidant enzymes increased firstly and then decreased. The maximum values of superoxide dismutase(SOD),catalase(CAT) and ascorbate peroxidase(APX) activities appeared in moderate drought stress, while that of peroxidase(POD)appeared in mild drought stress. Superoxide anion scavenging capacity(SASC), hydroxyl radical scavenging capacity(HRS) and total antioxidant capacity(T-AOC) increased firstly and then decreased. Under the condition of severe drought, the osmotic adjustment substances, antioxidant enzyme activities and antioxidant capacity of drought tolerant variety were significantly higher than those of sensitive variety. The membrane lipid peroxidation increased gradually with the degree of drought stress, and the sensitive variety had significantly higher value. The above studies showed that the adaptation and drought resistant ability of Heinong 44 were significantly greater than those of Heinong 65.更多还原