【摘要】 目的研究探讨EB病毒微小RNA在鼻咽癌中的表达情况,以便于找到诊断和防治鼻咽癌的生物标志物。方法选取45例鼻咽癌患者和37例鼻咽炎患者为研究对象,借助实时荧光定量PCR技术并以miR-U6作为内参物,对上述疾病病灶中的EB病毒miRNAs(包括EBV-miR-BART4*和EBV-miR-BART18-3P)的表达情况进行研究分析。结果经统计分析可知,EB病毒的两种miRNAs在鼻咽炎组织中的表达数值分别是(28.43±3.16)和(26.73±3.08),而在鼻咽癌组织中的表达数值分别是(40.51±4.82)和(41.66±3.46),可见其在鼻咽癌组织中的表达水平明显高于鼻咽炎组织,两者具有显著的差异性(P<0.01)。结论 EB病毒微小RNAs可通过较显著的表达水平导致鼻咽癌的发病和病灶转移,因此可将其作为鼻咽癌诊断防治中潜在的生物分子标志物。更多还原

【Abstract】 Objective To explore the expression of Epstein-Barr virus micro-RNA in nasopharyngeal carcinoma,and to discover the biomarker for diagnosis and treatment of nasopharyngeal carcinoma.Methods 45 patients with nasopharyngeal carcinoma and 37 patients with nasopharyngitis were chosen as the research objects.The expression of Epstein-Barr virus microRNAs including EBV-miR-BART4* and EBV-miR-BART18-3 P was studied and referred with miR-U6 by means of real-time fluorescent quantitative PCR.Results Through statistical analysis of experimental data,the expression of Epstein-Barr virus microRNAs in nasopharyngitis were respectively(28.43 ± 3.16) and(26.73 ± 3.08),and of nasopharyngeal carcinoma were respectively(40.51 ± 4.82) and(41.66 ± 3.46).The expression of Epstein-Barr virus micro-RNAs in nasopharyngeal carcinoma were higher than that of nasopharyngitis,the difference was significant(P < 0.01).Conclusion Epstein-Barr virus micro-RNA can express and result in nasopharyngeal carcinoma at a significantly high level,which means it can be treated as a molecular biomarker in the diagnosis,prevention and treatment of nasopharyngeal carcinoma potentially.更多还原