【摘要】 对连翘内生真菌J8抗氧化活性物质的发酵提取工艺进行优化。通过单因素筛选,结合Plackett-Burman试验确定影响提取工艺的主要因素,以响应面中心组合法(CCD)拟合了最优组合。结果表明,发酵提取的最优工艺为:当乙酸铵浓度为5.855g/L、介质pH值为7.82、料液比为1∶9.35时,ABTS+·清除率预期值为93.31%。将pH值设为7.8,液料比和乙酸铵不变,在此条件下,J8代谢产物的ABTS+·清除率达到90.23%,与预期值接近。此结果对连翘内生真菌J8进一步分离和纯化其抗氧化性物质研究提供了基础资料。更多还原

【Abstract】 To optimize the fermentation-extraction process of the antioxidant of an endophytic fungus J8 from forsythia,the single-factor experimental and Plackett-Burman method were contributed to identify the most notable factors,then the central composite design of the response surface analysis(RSA)was used to fit the optimal combination.The optimum conditions were achieved as following,ammonium acetate=5.855 g/L,pH=7.82,solid-liquid ratio=1∶9.35.The desired value of the scavenging rate of ABTS+·free radical was 93.31%.For the actual experiment,as pH fixed at 7.8 and other factors as the same mentioned above,the scavenging rate reached 90.23%,which is similar to the desired value.The result provides the basic information about the further separation and purification of the antioxidants originated from an endophytic fungus J8 from forsythia.更多还原