Objective To isolate,culture and identify human Mycoplasma pneumoniae(MP) strains. Methods Mycoplasma pneumoniae strains were isolated from throat swabs and sputum samples of hospitalized children by using medium supplemented with antibiotics or filtration with filtering membrane to remove most of the fungi and bacteria,and the time for culture was optimized. The isolates were identified by PCR,genotyping as well as tests for culture characteristics,biochemical characteristics and serological characteristic...