节点文献

QUARTET突变四倍体拟南芥的获得与表型分析

Development and Phenotypic Analysis of Tetraploid Arabidopsis thaliana with QUARTET Mutation

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 何硕康罗泽伟

【Author】 HE Shuo-kang;LUO Ze-wei;Institute of Biostatistics,School of Life Science,Fudan University;

【通讯作者】 罗泽伟;

【机构】 复旦大学生命科学学院生物统计所

【摘要】 为了将四分子分析应用于多倍体遗传重组的研究,本研究从qrt1基因突变的二倍体Columbia(Col)与Landsberg erecta(Ler)生态型拟南芥出发,利用秋水仙素诱导核基因组加倍,利用流式细胞技术及荧光原位杂交技术筛选出成功加倍的qrt1基因突变的四倍体Col与Ler生态型拟南芥。并对其进行营养及生殖相关表型分析。首先qrt1突变抑制了侧根数量、基部侧枝数量以及莲座叶片面积。其次,四倍体莲座叶片面积、花粉、花药以及种子更大,侧根及茎基部分枝减少,抽薹时间推迟,每株果荚数与每果荚种子数更少,但种子发芽率都接近100%。倍性还对某些表型的影响具有背景依赖性,如对主茎分支数量、最终株高以及果荚长度的影响在Col与Ler生态型中表现不同。

【Abstract】 To apply tetrad analysis in the research on genetic recombination of polyploid,we treated the diploid Columbia(Col)from gene qrt1 mutation and Arabidopsis thaliana of Landsberg erecta(Ler)ecotype with colchicine for inducing the genome doubling. Theflow cytometry and fluorescence in situ hybridization were employed to screen the successfully-doubled tetraploid Col and Ler from gene qrt1 mutation. Then their vegetable and reproductive phenotypes were analyzed. As results,firstly,qrt1 mutation reduced the number of lateralroots and side bolts and the area of rosette leaves. Secondly,the area of rosette leaves and the size of pollen,anther and seed of the tetraploidincreased,and the number of lateral roots and side bolts decreased,and the time of bolting siliques delayed,And the number of fruit pods perplant and seeds per fruit pod were less,while the germination rate approached 100%. Thirdly,some phenotypes were influenced differently byploidy in Col and Ler,for example,the effects of ploidy on the height of stem,the number of branches and length of siliques varied.

  • 【文献出处】 生物技术通报 ,Biotechnology Bulletin , 编辑部邮箱 ,2018年07期
  • 【分类号】Q943.2
  • 【被引频次】1
  • 【下载频次】247
节点文献中: 

本文链接的文献网络图示:

本文的引文网络