[Objective]Isolation and identification of halophilic bacteria in ancient salt well to determine their compatible solutes and increase the synthesis. [Methods] Halophilic strain were isolated from the brine of salt well,and the morphology,physiology,biochemistry and 16 S rRNA were analyzed. The compatible solute was determined by high performance liquid chromatography( HPLC) and mass spectrometry( MS). Sodium glutamate was used as the sole carbon and nitrogen source to produce ectoine.[Results] The species ...