【摘要】 95%乙醇室温浸提,50℃减压浓缩后得到无醇水液,用乙酸乙酯除脂后,得到血三七的除脂醇提液。将此除脂醇提液滤膜过滤后,通过制备液相分离,分得一个单一组分,将该单一组分浓缩后结晶,得到单体化合物。通过质谱和核磁共振技术鉴定单体化合物的结构为岩白菜素。以维生素C作为阳性对照,采用清除DPPH自由基试验方法研究化合物的抗氧化活性。结果显示,岩白菜素清除DPPH自由基的抗氧化的体系终浓度IC₅₀为0.245μg/mL,低于维生素C的体系终浓度(IC₅₀为0.575μg/mL),表明岩白菜素具有较强的抗氧化活性。更多还原

【Abstract】 To investigate the active composition from the radix of Polygonum amplexicaule var. sinense, the roots were powered, extracted with ethanol. The compound was separated and purified by PHPLC, and identified as bengenin on the basis of spectral analysis. Then the antioxidant activity of bengenin was evaluated by DPPH radical scavenging assay. The bengenin from the radix of Polygonum amplexicaule var. sinense shows a strong antioxidant activity much higher than vitamin C.更多还原