【摘要】 目的探讨过氧乙酸-乙醇(PES)联合辐照灭菌对脱钙骨基质(DBM)成骨诱导活性的影响。方法取SD大鼠长管骨制作成骨粉装入胶囊后分为两组进行消毒灭活处理:PES+20k Gy的钴-60射线消毒组(实验组)和20k Gy钴-60射线消毒组(对照组)。将DBM骨胶囊植入40只SD大鼠体内,分别在2、4、6、8周处死SD大鼠,将之前植入的DBM骨胶囊取出,对DBM的骨诱导活性进行检测。结果从大体观察看,实验组所植DBM骨块呈散在颗粒状、形态不完整,对照组所植DBM骨块呈卵圆形、质地较硬、形态完整;从新生微血管数(MVQ)看,实验组MVQ值少于对照组,差异有统计学意义(<0.0001);从新生骨生长速率看,对照组的生长速率为1.59 m/d,而实验组生长速率过低,无法测量;从钙、磷、碱性磷酸酶含量看,实验组钙含量小于对照组,差异有统计学意义(<0.05),实验组磷含量小于对照组,差异有统计学意义(<0.001),实验组碱性磷酸酶含量小于对照组,差异有统计学意义(<0.05)。结论使用PES联合辐照灭菌以后会降低DBM的成骨诱导活性。更多还原

【Abstract】 Objective To investigate the osteogenic activity of demineralized bone matrix(DBM) after Peracetic Acid-Ethanol(PES) combined irradiation sterilization. Methods The long bone of SD rats were divided into two groups to disinfection and inactivation: the experimental group which used PES solution add the cobalt-60 radiation at 20 k Gy,and the control group just only used cobalt-60 radiation at 20 k Gy. We made the DBM bone meal into capsules after disinfection, and implanted into SD rats, and the SD rats were sacrificed at 2, 4, 6, 8 weeks, and remove the plant DBM bone from SD rat to detect the bone induction activity. Results From general observation, the DBM bone fragments from the experimental group were planted incomplete, scattered particles, on the contrary, experimental group disinfection planting had hard texture and complete shape. From new bone quantitative analysis, the results shows that the control group sterilized osteogenic activity is much better than the experimental group. From analysis of the sample of calcium,phosphorus, alkaline phosphatase enzyme content, the control group content is more than experimental group. Conclusion The osteogenic induction activity of DBM was reduced by using PES combined irradiation sterilization.更多还原