【摘要】 目的:采用加压毛细管电色谱法测定复方芦丁片中芦丁与维生素C的含量。方法:采用Trisep^TM-2100加压毛细管电色谱仪,以C18毛细管色谱柱（300 mm×100μm i.d.,1.8μm）为固定相,流动相为甲醇-乙腈-0.4%磷酸（18:70:12）,用三乙胺调节pH值至3.0,总流速为0.05 mL·min^-1,检测波长为254 nm。结果:芦丁的线性范围为2.0²0μg·mL^-1（r²=0.999）;平均回收率为99.4%,RSD为1.2%（n=9）;维生素C的线性范围为2.0⁵0μg·mL^-1（r²=0.999）;平均回收率为99.3%,RSD为1.4%（n=9）。另外,本方法与原国标方法进行了比较,结果基本一致。结论:加压毛细管电色谱法可用于复方芦丁片有效成分含量的测定,该方法简单、快速、准确,为复方芦丁片质量控制提供了新检测技术。更多还原

【Abstract】 Objective: A pressurized capillary electrochromatography（p CEC） method has been developed for determination of the active components in compound rutin tablets. Methods: The separation was carried out on a 300 mm×100 μm i.d. capillary packed with1.8 μm ODS by using methanol-acetonitrile-0.4% phosphoric acid（18:70:12） as the mobile phase, and the pH was adjusted to 3.0 with triethylamine. The total flow rate was 0.05 mL·min^-1. The UV absorption detection was carried out at 254 nm. Results: The linear ranges for rutin and vitamin C were 2.0²0 μg·mL^-1（r²=0.999） and 2.0⁵0 μg·mL^-1（r²=0.999）, respectively. Mean recoveries of rutin and vitamin C were 99.4% with relative standard deviation 1.2%（n=9）, and 99.3% with relative standard deviation 1.4%（n=9）, respectively.Besides, the method was compared with the original GB method, the results were basically identical. Conclusions: The method can be used for the determination of compound rutin tablets, which is simple, rapid and accurate. It provides a new detection technology for the quality control of compound rutin tablets.更多还原