为进一步确认及掌握鲤浮肿病毒(Carp Edema Virus,CEV)目前的流行现状和流行特点,于2017年重点调查了我国北方地区5省市26个正发生或曾经发生鲤急性烂鳃病的养殖场,随机监测了9个省市的97家鲤和锦鲤养殖场。样品采用实时荧光定量PCR(Quantitative real time PCR,q PCR)法和套式PCR(Nest PCR)方法进行检测,nest PCR法扩增出的产物进行测序和基因分析。结果重点调查的26家养殖场中有20个为CEV阳性,1个为锦鲤疱疹病毒(Koi Herpes Virus,KHV)阳性,1个为孢子虫感染。随机调查的97家养殖场中有50家为CEV检测阳性,阳性率高达52%;可测序的CEV毒株均属于GenogroupⅡa型;样品按不同地区、不同采样水温、不同品种、不同规格鱼等划分,各组均有CEV阳性检出,但检出率均无显著差异。以上结果表明CEV感染是多省市鲤急性烂鳃病暴发的主要病因。另外,该病毒病的流行水温在12—27℃。
【英文摘要】
In 2017, to further confirm the speculation and epidemiological traits of carp edema virus(CEV), 26 carp farms in 5 provinces of north China, where acute rot gills disease of carp, were occurred, surveyed, and sampled.CEV and koi herpes virus(KHV) were detected using quantitative polymerase chain reaction(q PCR) and nested PCR techniques. In addition, 97 carp farms in 9 provinces of China were randomly surveyed for CEV and PCR products for sequencing and analyzing viral DNA of CEV. The results showed that 2...