HSJ296, a Scenedesmus strain isolated and purified by this lab, can grow heterotrophically in the dark and contains a relatively high amount of α-linolenic acid. Comparisons of heterotrophic growth at different temperatures and concentrations of glucose, or with different nitrogen sources, indicated that the optimal culture conditions included30℃, 4 g/L urea and 20—40 g/L glucose. As shown with fatty acid composition analyses under different conditions,the fatty acids of HSJ296 were mainly composed of hexad...