【摘要】 制备一种安全有效,口感好的翅果油亚微乳,并探讨翅果油及其亚微乳抗疲劳活性及机理。以翅果油为原料,通过对乳化剂等组分进行处方筛选、采用正交设计进行工艺优化,制备翅果油亚微乳制剂,并对翅果油及其亚微乳抗疲劳活性进行研究。分别进行小鼠负重游泳实验,记录其力竭游泳时间,测定血乳酸(blood lactic acid,BLA)、血清尿素氮(serum urea nitrogen,BUN)和肝糖原(hepatic glycogen,LG)等指标含量,计算BLA、BUN的清除速率及LG的储备量。翅果油亚微乳的最优处方为:混合乳化剂(卵磷脂、聚甘油脂肪酸酯、单硬脂酸甘油酯)为4%、翅果油5%、甘油为4%,蒸馏水为76%,山梨酸钾为1%,蔗糖为10%。与阴性对照组比较,翅果油中剂量组负重游泳时间、BLA、BUN、LG等指标均具有统计学差异(P<0.05):高、低剂量组负重游泳时间及三项生化指标的均值略高于阴性对照组。亚微乳组各项指标均优于阴性组、翅果油低、高剂量组。成功研制出一种新型的,吸收好,生物利用度高的翅果油亚微乳。翅果油及其亚微乳具有一定的提高运动耐力,抑制运动后血乳酸和尿素氮积累、增加肝糖原储备的作用,从而达到缓解运动性体力疲劳的作用。更多还原

【Abstract】 The safe,effective and good tastes elaeagnus mollis oil submicron-emulsion was prepared.Furthermore,to study the anti-fatigue activity of elaeagnus mollis oil and its submicron-emulsion. The formulation of elaeagnus mollis oil submicron-emulsion was optimized by orthogonal-design,and the individual components include the emulsifier and the co-emulsifier for preparing are subjected to single factor screening.After treatment,the mice were subjected to weight-bearing swimming test. The blood lactic acid(BLA),serum urea nitrogen(BUN) and hepatic glycogen(LG) were measured immediately after swimming. The best formulation of the submirco-emulsion was as follows:mix emulsifier was 4 %,oil was 5 %,glycerol was 4 %,distilled water 76 %,potassium sorbate 1 % and sucrose 10 %. The loading swim time and the levels of BLA,BUN,LG in middle dose group were remarkably changed when compared with that of the control group(P<0.05). While there was no significant difference in the loading swim time and three biochemical indicators between the high/low-dose group and the control group. The clearance ratio of BLA and BUN in middle dose group were apparently higher than the control group. Conclusion:A new,safe,high bioavailability of elaeagnus mollis oil submicron-emulsion was developed successfully. Elaeagnus mollis oil and its submicron-emulsion has the effect of inhibiting the accumulation of BLA and BUN after exercise,increasing the content of LG,so as to alleviating the physical fatigue of mice. This work would provide a new perspective on developing of elaeagnus mollis oil.更多还原