本研究利用亚临界水提取、可见分光光度法测定总黄酮含量及其抗氧化活性,并利用响应面法优化了怀菊花中总黄酮的亚临界水提取工艺。首先进行了单因素实验,然后在单因素实验基础上,选择提取温度、提取时间及液固比为自变量,以总黄酮提取量为响应值,采用Box-Benhnken法设计3因素3水平响应面设计实验,得出最佳工艺:提取温度130℃、提取时间46 min、液固比44∶1 m L/g,总黄酮实际提取量为(71.796±0.24)mg/g,与预测值71.806 mg/g无显著差异。结果表明,怀菊花中的总黄酮有一定的抗氧化活性,并且其含量与抗氧化活性具有一致性。
【英文摘要】
In this study,the total flavonoids from Huai Chrysanthemum were extracted by subcritical water and examined by visible spectrophotometry,while antioxidant activity of the total flavonoids was also determined,and the response surface method was used to optimize subcritical water extraction of total flavonoids in Huai Chrysanthemum.Firstly,single factor experiment was investigated,and then based on single factor experiment,a 3-factor,3-level Box-Benhnken experiment was designed,in which extraction temperature...
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