The two traditional glycosylation grafting methods and the improved methods were used to compare with the glycosylation product conformation and functional properties of the product,in order to further reveal the effect of protein glycosylation grafting methods on the process of glycosylation reaction and the product mechanism. The results showed,the aqueous phase system had a greater effect on the spatial structure of the protein than that of the solid phase system. In the secondary structure,the content o...
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